After maturation and germination of the transgenic somatic embryos, the plants were micropropagated and acclimated to ex vitro conditions. Three transgenic lines expressing the AtNPR1 gene under the control of the constitutive promoter CaMV35S were obtained using an embryogenic line derived from a seed of cv. The aim of this research was to generate transgenic olive plants expressing the NPR1 gene from Arabidopsis thaliana to evaluate their differential response to the hemibiotrophic fungus Verticillium dahliae and the necrotroph Rosellinia necatrix. The NPR1 gene has also been shown to be involved in the crosstalk between SAR signaling and the jasmonic acid-ethylene (JA/Et) pathway, which is involved in the response to necrotrophic fungi. Overexpression of NPR1 confers resistance to biotrophic and hemibiotrophic fungi in several plant species. The NPR1 gene encodes a key component of systemic acquired resistance (SAR) signaling mediated by salicylic acid (SA). 4Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones CientГficas, Avenida MenГ©ndez Pidal s/n, Campus de Excelencia Internacional Agroalimentario ceiA3, CГіrdoba, Spain.3Departamento de AgronomГa, College of Agriculture and Forestry (ETSIAM), Universidad de CГіrdoba, Campus de Excelencia Internacional Agroalimentario ceiA3, CГіrdoba, Spain.

2Departamento de GenГіmica y BiotecnologГa, Fruticultura Subtropical y MediterrГЎnea (IFAPA), Unidad Asociada de I+D+i al CSIC, MГЎlaga, Spain.1Instituto de Hortofruticultura Subtropical y MediterrГЎnea вЂњLa MayoraвЂќ, Departamento de BotГЎnica y FisiologГa Vegetal, Universidad de MГЎlaga, MГЎlaga, Spain.Trapero-Casas 4, Carlos Lopez-Herrera 4, Juan M. Isabel NarvГЎez 1, Clara Pliego Prieto 2, Elena Palomo-RГos 1, Louis Fresta 1, Rafael M.

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